28041 human pericytes promocell  (PromoCell)

Journal: bioRxiv

Article Title: Light-driven repair: Photobiomodulation restores blood–brain barrier function following hypoxic injury

doi: 10.64898/2026.02.15.706027

Figure Lengend Snippet:

Article Snippet: The cell lines used were purchased from Innoprot: human brain microvascular endothelial cells (HBMECs) (Innoprot; P10361-IM), human astrocytes (HAs) (Innoprot; P10251-IM), and human brain vascular pericytes (HVPCs) (Innoprot; P10363-IM).

Techniques: Staining

Journal: bioRxiv

Article Title: Light-driven repair: Photobiomodulation restores blood–brain barrier function following hypoxic injury

doi: 10.64898/2026.02.15.706027

Figure Lengend Snippet: (A-C) Quantification of ROS levels measured by luminescence assay in endothelial cells (A), astrocytes (B), and pericytes (C) following hypoxia (6 h, 1% O₂) with or without PBM treatment. PBM significantly reduced hypoxia-induced ROS accumulation in astrocytes and pericytes, with a modest effect in endothelial cells. (D-F) Representative ICC images showing HIF-1α (cyan) and nuclei (Hoechst, blue) in endothelial cells (D), astrocytes (E), and pericytes (F) under normoxia (–PBM), hypoxia (–PBM), and hypoxia with PBM (+PBM). Insets display higher-magnification views illustrating nuclear localisation of HIF-1α. The mRNA expression of HIF-1α was normalised to RPL13A. Nuclear HIF-1α protein levels were quantified as mean nuclear fluorescence intensity normalised to Hoechst area and expressed relative to normoxic controls. Statistical comparisons were performed using one-way ANOVA with Šídák’s post hoc correction; N=3–4 independent biological replicates. Data are presented as mean±SEM.

Article Snippet: The cell lines used were purchased from Innoprot: human brain microvascular endothelial cells (HBMECs) (Innoprot; P10361-IM), human astrocytes (HAs) (Innoprot; P10251-IM), and human brain vascular pericytes (HVPCs) (Innoprot; P10363-IM).

Techniques: Luminescence Assay, Expressing, Fluorescence

Journal: bioRxiv

Article Title: Light-driven repair: Photobiomodulation restores blood–brain barrier function following hypoxic injury

doi: 10.64898/2026.02.15.706027

Figure Lengend Snippet: (A,C,E) Average luminescence signal quantifying ROS levels in normoxic endothelial cells (A), astrocytes (C), and pericytes (E), comparing untreated and PBM-treated groups. (B,D,F) Representative ICC images of HIF-1α (cyan) with Hoechst nuclear counterstain (blue) in normoxic endothelial cells (B), astrocytes (D), and pericytes (F) under non-PBM (–PBM) and PBM-treated (+PBM) conditions. Quantitative comparisons were performed using one-way ANOVA with Šidák’s multiple comparisons test (N=4 biological replicates). Data are presented as mean ± SEM.

Article Snippet: The cell lines used were purchased from Innoprot: human brain microvascular endothelial cells (HBMECs) (Innoprot; P10361-IM), human astrocytes (HAs) (Innoprot; P10251-IM), and human brain vascular pericytes (HVPCs) (Innoprot; P10363-IM).

Techniques:

Journal: bioRxiv

Article Title: Light-driven repair: Photobiomodulation restores blood–brain barrier function following hypoxic injury

doi: 10.64898/2026.02.15.706027

Figure Lengend Snippet: (A–C) Mitochondrial oxygen consumption rate (OCR) profiles measured 24 hours after hypoxia (6 h, 1% O₂) in in endothelial cells (A), astrocytes (B), and pericytes (C), with or without PBM treatment. OCR traces are plotted as percentage change over time, normalised to protein content and baseline respiration. Sequential injections of oligomycin and FCCP were used to determine bioenergetic parameters. Basal respiration was calculated prior to oligomycin injection (pmol/min/µg protein). ATP-linked respiration was determined as the reduction in OCR following oligomycin and expressed relative to baseline OCR. Maximal respiration was calculated following FCCP treatment and normalised to OCR. Hypoxia significantly reduced mitochondrial function in endothelial cells and astrocytes, whereas PBM selectively increased maximal respiratory capacity in endothelial cells under hypoxic conditions. Statistical comparisons were performed using one-way ANOVA with Šídák’s post hoc correction; N=4 independent biological replicates. Data are presented as mean±SEM.

Article Snippet: The cell lines used were purchased from Innoprot: human brain microvascular endothelial cells (HBMECs) (Innoprot; P10361-IM), human astrocytes (HAs) (Innoprot; P10251-IM), and human brain vascular pericytes (HVPCs) (Innoprot; P10363-IM).

Techniques: Injection

Journal: bioRxiv

Article Title: Light-driven repair: Photobiomodulation restores blood–brain barrier function following hypoxic injury

doi: 10.64898/2026.02.15.706027

Figure Lengend Snippet: (A–F) Oxygen consumption rate (OCR) measurements in endothelial cells (A,D), astrocytes (B,E), and pericytes (C,F) 48 hours post-treatment. OCR traces are plotted over time and normalised to protein content and baseline respiration. Basal respiration (pmol/min/µg protein) was calculated prior to oligomycin injection. ATP-linked respiration was determined as the difference between basal respiration and OCR following oligomycin, normalised to total OCR. Maximal respiration was calculated from OCR after FCCP injection and normalised accordingly. Proton leak was derived from OCR after oligomycin injection, and spare respiratory capacity was defined as the difference between maximal and basal respiration, both normalised to OCR. Statistical comparisons were performed using one-way ANOVA with Šidák’s multiple comparisons test (N=4 biological replicates; *p<0.05, **p<0.01). Data are presented as mean ± SEM.

Article Snippet: The cell lines used were purchased from Innoprot: human brain microvascular endothelial cells (HBMECs) (Innoprot; P10361-IM), human astrocytes (HAs) (Innoprot; P10251-IM), and human brain vascular pericytes (HVPCs) (Innoprot; P10363-IM).

Techniques: Injection, Derivative Assay

Journal: bioRxiv

Article Title: Light-driven repair: Photobiomodulation restores blood–brain barrier function following hypoxic injury

doi: 10.64898/2026.02.15.706027

Figure Lengend Snippet: (A-C) Quantification of proton leak and spare respiratory capacity 24 hours after treatment in endothelial cells (A), astrocytes (B), and pericytes (C). Proton leak was calculated from OCR values following oligomycin injection and normalised to total OCR. Spare respiratory capacity was determined as the difference between maximal respiration (post-FCCP) and basal respiration, normalised to OCR. Statistical comparisons were performed using one-way ANOVA with Šidák’s multiple comparisons test (N=4 biological replicates; **p<0.01). Data are presented as mean ± SEM.

Article Snippet: The cell lines used were purchased from Innoprot: human brain microvascular endothelial cells (HBMECs) (Innoprot; P10361-IM), human astrocytes (HAs) (Innoprot; P10251-IM), and human brain vascular pericytes (HVPCs) (Innoprot; P10363-IM).

Techniques: Injection

Journal: bioRxiv

Article Title: Light-driven repair: Photobiomodulation restores blood–brain barrier function following hypoxic injury

doi: 10.64898/2026.02.15.706027

Figure Lengend Snippet: (A–C) ECAR measurements in endothelial cells (A), astrocytes (B), and pericytes (C) at 24 and 48 hours across all experimental conditions. Basal glycolysis (mpH/min/µg protein) was calculated prior to oligomycin injection. Glycolytic reserve capacity was defined as the difference between ECAR following oligomycin and basal glycolysis, normalised to total ECAR. Statistical comparisons were performed using one-way ANOVA with Šidák’s multiple comparisons test (N=4 biological replicates; *p<0.05, **p<0.01). Data are presented as mean ± SEM.

Article Snippet: The cell lines used were purchased from Innoprot: human brain microvascular endothelial cells (HBMECs) (Innoprot; P10361-IM), human astrocytes (HAs) (Innoprot; P10251-IM), and human brain vascular pericytes (HVPCs) (Innoprot; P10363-IM).

Techniques: Injection

Journal: bioRxiv

Article Title: Light-driven repair: Photobiomodulation restores blood–brain barrier function following hypoxic injury

doi: 10.64898/2026.02.15.706027

Figure Lengend Snippet: (A) Rgs5 mRNA expression in human vascular pericytes (HVPCs) at 48 hours across all experimental conditions, normalised to RPL13A. (B) Representative ICC images showing PDGFRβ (yellow) and nuclei (Hoechst, blue) in normoxic and hypoxic HVPCs, with and without PBM treatment. (C) Quantification of PDGFRβ protein expression relative to control, calculated as mean fluorescence intensity normalised to Hoechst area. (D) αSMA mRNA expression in HVPCs at 48 hours, normalised to RPL13A. (E) Representative ICC images showing filamentous actin (magenta) and nuclei (blue) under normoxic and hypoxic conditions ± PBM. (F) Quantitative analysis of actin protein expression relative to control, measured as mean fluorescence intensity normalised to Hoechst area. Statistical comparisons were performed using one-way ANOVA with Šidák’s multiple comparisons test (N=3–4 biological replicates). Data are presented as mean ± SEM.

Article Snippet: The cell lines used were purchased from Innoprot: human brain microvascular endothelial cells (HBMECs) (Innoprot; P10361-IM), human astrocytes (HAs) (Innoprot; P10251-IM), and human brain vascular pericytes (HVPCs) (Innoprot; P10363-IM).

Techniques: Expressing, Control, Fluorescence

Journal: Materials Today Bio

Article Title: Redirecting the route: Monocyte-mediated delivery of oHSV-1 across a human BBB-on-chip model

doi: 10.1016/j.mtbio.2025.102458

Figure Lengend Snippet: Microfluidic BBB-on-chip design and validation. A. Schematic representation of the BBB-on-chip, seeded with endothelial cells on the blood side and pericytes and astrocytes on the brain side, equipped with three microwells for 3D GBM spheroid culture (GBM spheroids area). B. Image of the assembled BBB-on-chip showing the upper channel (yellow) mimicking the vascular compartment and main perfusion conduit, and the lower channels (red) leading to the tumor compartment (scale bar = 2 cm). C. Transendothelial electrical resistance (TEER) measurements recorded daily across the membrane between the upper and lower channels and supporting the BBB. D. Permeability coefficients calculated from the diffusion of 40, 70, and 150 kDa FITC-dextrans through the assembled BBB-on-chip in the following conditions: i. No cells (control, membrane without cells), ii. Blood side (membrane + HUVECs endothelial monolayer), and iii. BBB complete (membrane with the three-culture of HUVECs on the blood side and pericytes and astrocytes on the brain side). E . IF staining of the BBB-on-chip model on day 7. On the blood side compartment , endothelial tight junction protein ZO-1 (green) and the endothelial marker CD31 (green) are expressed, confirming the formation of a continuous endothelial layer. On the brain side , astrocytes are identified by GFAP expression (magenta), indicating appropriate localization. DAPI (blue) highlights cell nuclei in both compartments. Scale bar = 50 μm. Values are expressed as median±SEM from at least 3 independent experiments. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001 vs control. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The assembled BBB-on-chip devices were bound to glass coverslips via further plasma treatment and sterilized with 70 % ethanol followed by UV exposure for 60 min. BBB-on-chip cells seeding and culturing: Human umbilical vein endothelial cells (HUVECs), brain vascular pericytes (hBVPs), and immortalized astrocytes (hAs) were obtained from Innoprot (Derio, Spain).

Techniques: Biomarker Discovery, Membrane, Permeability, Diffusion-based Assay, Control, Staining, Marker, Expressing